Journal: Translational Psychiatry

Article Title: Polyunsaturated fatty acid deficiency during neurodevelopment in mice models the prodromal state of schizophrenia through epigenetic changes in nuclear receptor genes

doi: 10.1038/tp.2017.182

Figure Lengend Snippet: Effects of ligands for nuclear receptors in vitro and in vivo . ( a ) Top row: schematic of the experiment using OLP6 cells. During the first 4 days, the cells were cultured in an undifferentiated condition. During the subsequent 4 days, the cells were cultured in a differentiated condition. On day 7, each ligand was added. On day 8, the transcript levels were measured by quantitative RT-PCR. Bottom row: quantitative RT-PCR analysis of oligodendrocyte-related genes in OLP6 cells treated with 0.1, 0.3 or 1 μ m bexarotene or 5, 15 or 50 μ m bezafibrate. This examination was performed in triplicate. ( b ) Top row: schematic of the experiment using KATO-III cells. Bottom row: gene expression of CCK and CALB2 in KATO-III cells treated with 0.1, 0.3 or 1 μ m bexarotene or 5, 15 or 50 μ m bezafibrate and analyzed by quantitative RT-PCR. Values are means±s.e. Gapdh / GAPDH was used as an internal control. P -values were calculated using Dunnett’s test for multiple comparisons (compared with DMSO). This examination was performed in triplicate. ( c ) Top row: experimental design for the administration of bexarotene. Mice received daily injections from 6 to 9 weeks of age (vehicle or bexarotene, 30 or 100 mg kg −1 , once per day). Bottom row: gene expression of Olig2 and Apc in mouse prefrontal cortexes (PFCs) analyzed by quantitative RT-PCR. Values are means±s.e. Gapdh was used as an internal control. P -values were calculated using Dunnett's test for multiple comparisons (compared with vehicle). Note that 30 mg kg −1 bexarotene administration elicited a significant increase in the expression of Olig2 and Apc . ( d , e ) Locomotor responses to injection of MK-801 (0.3 mg kg −1 ) in the vehicle- and bexarotene (30 mg kg −1 )-pretreated mouse groups. For both groups, the last treatment was administered 180 min before MK-801 injection. Values are means±s.e. P -values were calculated using two-way repeated measures ANOVA followed by post hoc Mann–Whitney U test d . P -values were calculated using unpaired t- tests e . # P <0.1, * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.

Article Snippet: OLP6 cells (RIKEN BioResource Center Cell Bank, Tsukuba, Japan) were incubated in a humidified atmosphere with 5% CO 2 at 33 °C in the proliferative stage.

Techniques: In Vitro, In Vivo, Cell Culture, Quantitative RT-PCR, Gene Expression, Control, Expressing, Injection, MANN-WHITNEY

Journal: Frontiers in Nutrition

Article Title: An Industrial and Sustainable Platform for the Production of Bioactive Micronized Powders and Extracts Enriched in Polyphenols From Olea europaea L. and Vitis vinifera L. Wastes

doi: 10.3389/fnut.2020.00120

Figure Lengend Snippet: Polyphenolic composition of olive leaves powders OLP1 and OLP2 after extraction, membrane purification, under vacuum concentration, and final dryer procedure.

Article Snippet: OLP1 and OLP2 powders, produced from Frantoio and Leccino leaves, using membrane purifications and vacuum concentrations, show different polyphenol concentration whose main component is OLE, as reported in .

Techniques: Extraction, Membrane, Purification, Concentration Assay

Journal: Advanced Pharmaceutical Bulletin

Article Title: Novel Methotrexate-Ciprofloxacin Loaded Alginate-Clay Based Nanocomposite as Anticancer and Antibacterial Co-Drug Delivery System

doi: 10.34172/apb.2021.055

Figure Lengend Snippet: Results ofEDX elemental analysis of the OLP, OLPALg, OMT and OMTALg nanocomposites

Article Snippet: TGA of Laponite-RD, montmorillonite, OLP, OMT, OLPALg and OMTALg nanocomposites were performed with a Mettler-Toledo model 822 instrument.

Techniques: